Fig. 5. HOTAIR knockdown increases the 5hmC levels in SOX17 and MAGI2 promoters in HeLa cells. HeLa cells were transiently transfected with DsiCTRL or DsiHOTAIR, and the hydroxymethylation level in PCDH10 (A), SOX17 (B), AJAP1 (C) and MAGI2 (D) promoters was determined 48 hours later as described in the methodology. For each gene shown: the transcription start site (black arrow), CpG island (red box), TET1 binding sites (black line), the amplified region in the qPCR, CCGG site (red sequence) and a binding site for transcription factors related to 5hmC. The bars represent the mean ± standard deviation from at least three independent experiments. *p˂0.05.